California Institute of Technology Inter-Office Memo
To: Prof. Pauling
Subject: N. Terminal Sequences of Normal Adult Human Hemoglobin
As you know, the N-terminal sequence of the A chains is val-leu. Herb has now been able to identify the N-terminal sequence
of the B chains. He has isolated a dipeptide, val-his, and a tripeptide, val-his, leu. Quantitatively, they account for about
1 ½ of the two B chains. The tripeptide may be isolated in about twice the quantity of the dipeptide when a 15-minute hydrolysis
in refluxing 6 N HCl is used.
I thought that this information might [sic] of interest to you and might be of use at the Protein Conference in Paris.
Hirs etc & Anfinson Mention. 1F NaCl + 4F urea --> 1.8000 Hb."